Identification of a structural gene encoding a metallothionein-like domainthat includes a putative regulator protein for Streptomyces protease gene expression

Authors
Taguchi, S Ogawa, T Endo, T Momose, H
Citation
S. Taguchi et al., Identification of a structural gene encoding a metallothionein-like domainthat includes a putative regulator protein for Streptomyces protease gene expression, BIOS BIOT B, 62(12), 1998, pp. 2476-2479
Citations number
13
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
62
Issue
12
Year of publication
1998
Pages
2476 - 2479
Database
ISI
SICI code
0916-8451(199812)62:12<2476:IOASGE>2.0.ZU;2-6
Abstract
An open reading frame (termed ORF-PR) encoding a metallothionein-like domai n-including protein was found upstream of a previously identified Streptomy ces chymotrypsin-type protease gene (sam-P20). Promoter and terminator acti vities of ORF-PR were detected using the promoterless Streptomyces tyrosina se gene as a reporter gene and expression of ORF-PR was supposed to occur b efore that of sam-P20 gene. Frameshift mutation anaysis showed that the ORF -PR product might act as a repressive regulator of the sam-P20 gene.