Class I typing by PCR-SSP in Olomouc

In this article, methodical experience and population data are given for ap plication of PCR-SSP/ARMS method (Phototyping(1)) to HLA class I typing in Olomouc. Phototyping is reliable method suitable for typing of small to med ium number of samples. Method is fast enough for use in on-call service, re solution is better than the level of good serology, and price of method is comparable with serology. Our experience and tips are described below. Popu lation data of healthy unrelated individuals for HLA-A, -B, -Cw are given i n the tables.