E. Cecuk-jelicic et al., Implication of molecular analysis of HLA-A*02 subtyping for unrelated bone-marrow donor selection, BONE MAR TR, 22, 1998, pp. S27-S30
Citations number
17
Categorie Soggetti
Hematology,"Medical Research Diagnosis & Treatment
The HLA-A*02 allele is the most heterogeneous allele at HLA-A locus with 22
different subtypes so far identified. All of these subtype polymorphisms a
re located in alpha 1 and alpha 2 domains which are responsible for peptide
biding and HLA restricted recognition by T-cell receptor.
The aim of the present study was to determine the frequency of different HL
A-A*02 alleles in 33 healthy unrelated Croatians. HLA-A*02 subtyping has al
so been retrospectively performed in 2 recipient-unrelated donor pairs and
in 4 recipient-HLA phenotypically identical parent pairs. All subjects, pre
viously typed as HLA-A2 by serology were tested using HLA-A*02 ARMS-PCR kit
which discriminates 17 different A*02 alleles.
Among 17 A*02 alleles we have found 4 different A*02 subtypes in healthy un
related Croatians. The most frequent A*02 allele was A*0201 (84%). The freq
uency of the remaining A*02 alleles were as follows: A*0205 (3%), A*0207 (6
%) and A*0213 (6%). Among 6 tested bone-marrow transplantation (BMT) pairs,
only one has been found to be A*02 subtype incompatible (A*0201/A*0205).
Four different A*02 alleles are found in Croatian population with the predo
minance of A*0201. However these results suggest that A*02 subtyping is als
o necessary for optimal matching of HLA-A*02 positive donor-recipient pairs
in HLA incompatible BMT.