Isolation and short term culture of canine adrenal microvascular endothelial cells

A rapid method for the isolation of endothelial cells from canine adrenal g lands is described. The technique is based on collagenase digestion of adre nal glands following mechanical tissue disruption. The endothelial cells at tach and spread onto plastic petri dishes coated with a commercial attachme nt factor approximately one hour after seeding. Duration to first passage w as approximately one week. Doubling times were 2-3 days. Cells were positiv ely identified with immunoperoxidase staining for von Willebrand factor ant igen.