Mm. Mboloi et al., Validation of the indirect MAP1-B enzyme-linked immunosorbent assay for diagnosis of experimental Cowdria ruminantium infection in small ruminants, CL DIAG LAB, 6(1), 1999, pp. 66-72
The major antigenic protein 1 fragment B (MAP1-B) enzyme-linked immunosorbe
nt assay (ELISA) for the diagnosis of Cowdria ruminantium infections was va
lidated to determine cutoff values and evaluate its diagnostic performance
with sheep and goat sera. Cowdria-infected populations consisted of 48 shee
p and 44 goats, while the noninfected populations consisted of 64 sheep and
107 goats. Cutoff values were determined by hue-graph receiver-operating c
haracteristic (TG-ROC) curves. The cutoff value was set at 31 and 26.6% of
the positive control reference samples for sheep and goat sera, respectivel
y. The test's diagnostic performance was evaluated with measurements of the
area under the concentration-time curve (AUC) of the ROC curves and by the
valid range proportion (VRP). The AUCs were 0.978 for sheep sera and 0.989
for goat sera. The VRP for both sheep and goat sera was approximately 1.0.
The intermediate range (IR), which defines results that are neither positi
ve nor negative, was 0 for goat sera and 2.81 for sheep sera. In an ideal t
est, the AUC and VRP would be 1.0 and the IR would be 0, In this study thes
e parameters were dose to those of an ideal test. It is concluded that the
MAP1-B ELISA is a useful test for the diagnosis of C. ruminantium infection
in small ruminants.