Protein detection using conducting polymer microarrays

An array of conducting polymer coated microelectrodes was employed as an am perometric detector to analyze a range of proteins. Using conducting polyme r coatings with different counterions incorporated, varying selectivity ser ies have been obtained. Protein identification and quantification were perf ormed using chemometric techniques such as principal component analysis (PC A), soft independent modelling of class analogy (SIMCA), ordinary least squ are (OLS) and partial least square (PLS). Using four different polymers, re sponse patterns were obtained and classification of six proteins was achiev ed. Individual proteins in a two component mixture were quantitatively anal yzed with acceptable accuracy.