Biodegradation patterns were measured for fresh and aged petroleum products
of similar chromatographic distribution in soil. An aerobic respirometer m
easured utilization of the petroleum products by indigenous soil microorgan
isms in soil slurries over a 40-day period. Hydrocarbon concentration chang
es in the soils were measured at various intervals over the 40 days using a
Diesel Range Organics (DRO) method. These tests were applied to two soils
with different physical characteristics, contamination profiles, and treatm
ent histories. Results indicated greater rates of hydrocarbon loss for the
soils containing the fresh petroleum product compared with the aged product
, and the lower molecular weight fraction of either product. The treatment
history of the two aged soils controlled their extent of further hydrocarbo
n loss. A simple biodegradability test may be an effective tool in screenin
g the potential success of bioremediation and estimating the final hydrocar
bon concentration achievable.