Effect of type XII or XIV collagen NC-3 domain on the human dermal fibroblast migration into reconstituted collagen gel

Citation
N. Akutsu et al., Effect of type XII or XIV collagen NC-3 domain on the human dermal fibroblast migration into reconstituted collagen gel, EXP DERMATO, 8(1), 1999, pp. 17-21
Citations number
20
Categorie Soggetti
Dermatology
Journal title
EXPERIMENTAL DERMATOLOGY
ISSN journal
09066705 → ACNP
Volume
8
Issue
1
Year of publication
1999
Pages
17 - 21
Database
ISI
SICI code
0906-6705(199902)8:1<17:EOTXOX>2.0.ZU;2-N
Abstract
Type XII and XIV collagens localize near the surface of banded collagen fib rils and most likely work as a molecular bridge between collagen fibrils. W e have shown that both collagens can modulate the interactions between coll agen fibrils, allowing fibroblasts to act upon the fibrils to vary the defo rmability. In the present study the effect of the globular domains (collage nase-resistant domains) of type XII and XIV collagens (XII-NC-3 and XIV-NC- 3) on the migration of fibroblasts into the reconstituted type I collagen g el was investigated. Cell attachment and proliferation on the collagen gel were unaffected. The migration of fibroblasts into the gel was increased pr oportionally to the concentration of collagen. We found that XII-NC-3 and X IV-NC-3 domains caused decreases in the numbers of fibroblasts that migrate d into the gel. Heat treatment of XII-NC-3 and XIV-NC-3 or the addition of polyclonal antibodies eliminated the suppressive activity on fibroblast mig ration, showing that the intact conformation of NC-3 domain is important fo r suppression of migration. The results suggest that both NC-3 domains infl uence the deformability of type I collagen fibril networks, which may cause the change in fibroblast migration.