Dexamethasone modulation of multidrug transporters in normal tissues

The expression of P-glycoprotein (P-gp) and canalicular multispecific organ ic anion transporter (cMOAT or,Mrp2) was evaluated by Western blotting anal sis of rat tissues isolated following daily administration (1 mg kg(-1) da y(-l)) of dexamethasone over 4 days, Dexamethasone rapidly increased P-gp e xpression more than 4.5- and 2-fold in liver and lung, respectively, while it If as decreased 40% in kidney. cMOAT expression was increased 2-fold in liver and kidney following dexamethasone treatment. The levels of both prot eins returned to control values by 6 days after the conclusion of dexametha sone administration, These results indicate that dexamethasone ran modulate P-gp and cMOAT expression in specific rat tissues and may have significant relevance for patients treated with desamethasone as a single agent or in combination therapy with other drugs. (C) 1999 Federation of European Bioch emical Societies.