Production and purification of lipase by a mutant strain of Rhizopus arrhizus

Authors
Chattopadhyay, M Banik, AK Raychaudhuri, S
Citation
M. Chattopadhyay et al., Production and purification of lipase by a mutant strain of Rhizopus arrhizus, FOL MICROB, 44(1), 1999, pp. 37-40
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
FOLIA MICROBIOLOGICA
ISSN journal
00155632 → ACNP
Volume
44
Issue
1
Year of publication
1999
Pages
37 - 40
Database
ISI
SICI code
0015-5632(1999)44:1<37:PAPOLB>2.0.ZU;2-U
Abstract
Extracellular lipase production by Rhizopus arrhizus was increased by mutan t selection from 130 to 670 mu mol FFA per mt per min using UV radiation an d aziridine treatment. The produced lipase was purified 720-fold by ammoniu m sulfate fractionation and Sephadex G-100 gel filtration. The molar mass o f the produced lipase was determined to be approximately 67 kDa which comig rated with bovine serum albumin in both a Sephadex G-100 column and SDS-PAG E.