Cytogenetic analysis of Malpighian tubule polytene chromosomes of Culex pipiens (Diptera : Culicidae)

A simple technique is described for obtaining well-spread and readable Malp ighian tubule polytene nuclei of Culex pipiens on a routine basis. Detailed polytene chromosome maps are presented with a description of the most prom inent landmarks of each chromosome, the regions with asynapsis and the most frequent weak points identified in the polytene arms. Usable Malpighian tu bule polytene chromosomes should facilitate molecular cytogenetic, genetic, and potentially biosystematic studies on this medically important global v ector of viral inducing encephalitis.