PTEN mutations in endometrial cancers with 10q LOH: Additional evidence for the involvement of multiple tumor suppressors

Objective. The aim of this study was to assess the involvement of PTEN and other putative 10q tumor suppressors in endometrioid-type adenocarcinomas c haracterized by loss of 100 sequences. Methods. PCR-based single-stranded conformational variant analysis and sequ encing of individual PTEN exons in 34 tumor specimens and their correspondi ng normal DNA were used. Results. Thirteen of the 34 tumors (38%) revealed a PTEN mutation: 2 frames hift, 3 nonsense, 3 missense, 3 splice site alterations, and 2 homozygous d eletions. Conclusion. The observation that greater than 60% of endometrial cancers wi th 10q LOH lack PTEN mutations, in addition to previously reported LOH data , provides evidence for the existence of other tumor suppressors on 10q. Co nsideration of PTEN mutation status may prove important in deletion mapping studies to locate additional tumor suppressors on the long arm of chromoso me 10. (C) 1998 Academic Press.