CA 125 is dominantly expressed by arrest of G0/G1 phase in uterine cervical adenocarcinoma cell line

Our purpose is to evaluate at which phase of the cell cycle CA 125 is domin antly expressed in a uterine cervical adenocarcinoma cell line (CAC-1). By a flow cytometric analysis of CA 125 expression, approximately 50% of the c ells treated with interferon-gamma (IFN) were positive after 24 h. In cell cycle, the G0/G1 arrest was observed in cells treated with IFN after 24 h. The pattern for the transition of the relative rate of CA 125 expression wa s similar to that of the population of G0/G1. Furthermore, in the G0/G1 pha se, the positive rate of CA 125 expression in cells treated with IFN was 80 % of the expression in all cells after 24 h. Additionally, flow cytometry w ith Ki-67 demonstrated that G1 arrest occurred at 24 h, and then G0 arrest was induced by IFN after 48 h of incubation. The increased expression of CA 125 appeared mainly in the G1 phase rather than in G0. (C) 1998 Academic P ress.