Cell-mediated immune response to bovine herpes virus (BHV-1) infection in experimental animals

Leucocyte migration inhibition (LMI) test was used to study the cell-mediat ed immune response in rabbits after experimental infection with BHV-1. The BHV-1 was propagated in MDBK cell line cultures with titre of 10(8.5) TCID5 0/ml and used for antigen preparation. The optimum concentration of antigen used was 100 mu g/ml for LMIT. Rabbits (10) divided into 2 groups (A and B ) of 5 each, including control were used. The peak LMI response was observe d 30th day post infection (PI) with 62.90% migration inhibition in group A and 25th day PI in group B with 60.59% migration inhibition. The overall LM I response showed by the rabbits of group A was not significantly different (P>0.05) from the rabbits of group B. However, there was significant posit ive correlation (P<0.005) between 2 groups.