PREPARATIVE DESALTING OF BOVINE SERUM-ALBUMIN BY CONTINUOUS ANNULAR CHROMATOGRAPHY

Bovine serum albumin (BSA) was continuously desalted from alkaline sal ts (a mixture of sodium phosphate, sodium chloride and potassium chlor ide) using a size-exclusion gel as the stationary phase. An annular ch romatograph was used to achieve a continuous mode of operation and the refore a reasonable throughput. Distribution and mass transfer coeffic ients of the substances as well as bed properties were obtained by bat ch chromatography. These separations were simulated mathematically app lying an approximate linear chromatographic theory. It was shown exper imentally and theoretically that the BSA and the salt solution could b e recovered continuously in a purity higher than 98%. The influence of rotation rate on the resolution of the individual peaks was investiga ted.