MONITORING OF SELECTED METABOLITES AND BIOTRANSFORMATION PRODUCTS FROM FERMENTATION BROTHS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Reversed-phase high-performance liquid chromatographic methods with UV -photodiode array or multiwavelength detection were developed for the analysis of secondary fungal metabolites mevinolin (also known as mona colin K, lovastatin or Mevacor) and fumagillin in fermentation broths. Samples obtained from screening experiments of Aspergillus fumigatus and Aspergillus terreus strains were analyzed for fumagillin and mevin olin, respectively. Isocratic and gradient elution was used with aceto nitrile-aqueous phosphoric acid or acetonitrile-phosphate buffer eluen ts. Sample preparation was accomplished by extraction of the whole fer mentation broth with an acid-organic solvent mixture. Similarly, bacte rial degradation products of 2,4,6-trichlorophenol obtained from micro bial remediation processes were analyzed by reversed-phase HPLC with U V-photodiode array detection. Extraction of the analyte from solid sam ples was done by solvent extraction and solid-phase extraction clean-u p using octadecyl-modified silica sample preparation cartridges.