SEPARATION OF PRIMARY AND SECONDARY-AMINES AS THEIR SULFONAMIDE DERIVATIVES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A series of simple and low-molecular-mass aliphatic amines were deriva tized with 8-quinolinesulfonyl chloride. Mixtures of the respective su bstituted sulfonamides were separated by reversed-phase high-performan ce liquid chromatography (RP-HPLC) and the sulfonamides were found to be highly sensitive to ultraviolet detection. This pre-column derivati zation facilitated good separation of eleven amines with the separatio n factors, alpha, better than 1.10 and the separation time within 20 m in. The optimization of separation was conducted and investigated on i ntermediate polarity (cyano) and apolar (hexyl) stationary phases. A l inear relationship was observed between the retention and the incremen tal carbon number of sulfonamides formed and thus making possible the retention prediction of the amine derivatives on RP-HPLC columns.