DETERMINATION OF MOLECULAR-MASS DISTRIBUTION OF FOOD-GRADE PROTEIN HYDROLYZATES BY SIZE-EXCLUSION CHROMATOGRAPHY AND CHEMILUMINESCENT NITROGEN DETECTION
Em. Fujinari et Jd. Manes, DETERMINATION OF MOLECULAR-MASS DISTRIBUTION OF FOOD-GRADE PROTEIN HYDROLYZATES BY SIZE-EXCLUSION CHROMATOGRAPHY AND CHEMILUMINESCENT NITROGEN DETECTION, Journal of chromatography, 763(1-2), 1997, pp. 323-329
Citations number
15
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Size-exclusion chromatography (SEC) and chemiluminescent nitrogen dete
ction (CLND) were used to estimate the molecular-mass distribution of
food-grade protein hydrolyzates. Simultaneous CLND and UV (214 nm) det
ection is demonstrated for analytical SEC of an experimental casein hy
drolyzate. In order to validate the estimated average M-r values deriv
ed from the SEC column data, a preparative SEC separation of an extens
ive casein hydrolyzate was pursued. Fractions were collected on a time
basis and analyzed by time-of-flight (TOF) mass spectrometry. A plot
of ToF M-r vs. SEC M-r indicated that the peptides below M-r of 1200 w
ere eluted as estimated by the calibrated preparative SEC column. This
paper demonstrates the power of using a dual CLND and UV detection sy
stem for analytical SEC analysis of protein hydrolyzates with a calibr
ated column.