PREPARATIVE SEPARATION OF ANTHOCYANINS BY GRADIENT ELUTION CENTRIFUGAL PARTITION CHROMATOGRAPHY

Centrifugal partition chromatography (CPC) allowed purification of ant hocyanins from Champagne vintage by-products (Vitis vinifera) and from blackcurrant (Ribes nigrum L.). In the first case a 5-1 pilot-CPC was used on a preparative multi-gram scale. The biphasic solvent system u sed was ethyl acetate-1-butanol-water, acidified by TFA (pH 1-3) for b oth gradient and isocratic normal-phase elutions. Separation selectivi ties which differ for cyanidin and delphinidin glycosides in CPC and c ellulose-TLC using the solvent system 1-butanol-acetic acid-water (4:1 :5) are discussed.