Targeted mutagenesis by duplication insertion in the radioresistant bacterium Deinococcus radiodurans: Radiation sensitivities of catalase (katA) andsuperoxide dismutase (sodA) mutants

Authors
Markillie, LM Varnum, SM Hradecky, P Wong, KK
Citation
Lm. Markillie et al., Targeted mutagenesis by duplication insertion in the radioresistant bacterium Deinococcus radiodurans: Radiation sensitivities of catalase (katA) andsuperoxide dismutase (sodA) mutants, J BACT, 181(2), 1999, pp. 666-669
Citations number
25
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
2
Year of publication
1999
Pages
666 - 669
Database
ISI
SICI code
0021-9193(199901)181:2<666:TMBDII>2.0.ZU;2-2
Abstract
Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method wa s developed to generate catalase (katA) and superoxide dismutase (sodA) mut ants. Both mutants were shown to be more sensitive to ionizing radiation th an the wild type.