Region specificity of chromosome III on gene expression in the yeast Saccharomyces cerevisiae

A single copy of a reporter gene cassette, such as PGKP-lacZ-LEU2 (promoter -reporter-marker gene) cassette, was inserted into one of 32 positions alon g chromosome III in Saccharomyces cerevisiae with an interval of approximat ely 10 kb. The amounts of translational gene product (beta-galactosidase) s ynthesized by the cassette-transformed cells were then determined. The regi on specificity in chromosome III could be demonstrated in gene expression: two higher-expressed regions (hot regions), 133 and 199 (MAT) regions, and seven lower-expressed regions (cold regions). For the steady and high produ ction of polypeptide, foreign gene products, by yeast, we would like to sta te that we hope for an insertion of the artificially prepared gene cassette [(promoter)-(foreign gene)-(marker gene)] into a hot region, such as 199 ( MAT) region of chromosome III.