Phospholipids hydrolysis in organic solvents catalysed by immobilised phospholipase C

Phospholipase C from Bacillus cereus has been immobilised on XAD7, Sepabead s FP-DA, Eupergit C, Celite 547, Silica gel 60 by covalent attachment or by adsorption. Preparates obtained with the two different immobilisation tech niques show good hydrolytic activity in water-saturated organic solvents wi th phosphatidylcholine (PC) as a substrate. The chiral 1,2-diacyl glycerol can easily be obtained from the organic phase. The catalysts obtained by co valent attachment have been prepared with a higher specific activity and ar e suitable for repeated uses, while the ones prepared by adsorption with lo wer specific activity can only be used once. The initial rates of hydrolysi s of PC solutions in different organic solvents and water content are compa red. (C) 1999 Elsevier Science B.V. All rights reserved.