Electrochemical behaviour of cytochrome c at electrically heated microelectrodes

The structural changes in cytochrome c with temperature have been been foll owed using a recently developed electrically-heated microelectrode sensor. Differential pulse voltammetry was used to perform electrochemical measurem ents of cytochrome c oxidation at different temperatures at heated bare gol d electrodes contained in phosphate-buffered cytochrome c solution at room temperature. The voltammetric response shows the onset of unfolding and a m arked dependence of the signal on electrode temperature. This augurs well f or applications of heated electrodes as local probes in the study of the te mperature dependence of electron transfer processes of other redox proteins , avoiding problems of bulk deterioration. (C) 1999 Elsevier Science B.V. A ll rights reserved.