Improving measurement stability and reproducibility of potentiometric sensors for polyions such as heparin

Citation
S. Mathison et E. Bakker, Improving measurement stability and reproducibility of potentiometric sensors for polyions such as heparin, J PHARM B, 19(1-2), 1999, pp. 163-173
Citations number
19
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN journal
07317085 → ACNP
Volume
19
Issue
1-2
Year of publication
1999
Pages
163 - 173
Database
ISI
SICI code
0731-7085(199902)19:1-2<163:IMSARO>2.0.ZU;2-D
Abstract
The growing importance of polymer membrane-based potentiometric polyion sen sors in biomedical research and clinical measurements has brought up the qu estion of how accurate and reproducible these sensors are. Indeed, recent r esearch has revealed that these sensors behave quite differently than class ical ion-selective electrodes. This paper explores ways to improve measurem ent reproducibility and long term potential stability by considering the un ique pseudo steady-state response mechanism of the polyion sensors develope d so far. Heparin may be stripped out of the phase boundary membrane surfac e with a high sample NaCl concentration and this characteristic is used to modify the calibration procedure in order to avoid memory effects. It is al so attempted to reduce long term potential drifts by continuously stripping heparin out of the membrane at the membrane-inner filling solution side. ( C) 1999 Elsevier Science B.V. All rights reserved.