Reversed-phase ion-pair HPLC determination of some water-soluble vitamins in pharmaceuticals

A reversed-phase ion-pair high-performance liquid chromatografic method (RP -IPC) was developed to assay some water-soluble vitamins in solution dosage forms. Vitamins of the B-group B-1, B-2, B-3, and B-6, including vitamin C were determined in Oligovit(R) coated tablets. In Beviplex(R) coated table ts the vitamins B-1, B-2, B-3, B-6 and p-aminobenzoic acid were analysed. H exanesulphonic acid sodium salt and triethanolamine in water-methanol were used as mobile phase with adjusting pH to 2.8 with orthophosphoric acid. Ph enol was used as an internal standard. For quantitative simultaneous analys is of vitamins in pharmaceutical formulations, the method of internal stand ard was used. All parameters for the validation of the method are given. (C ) 1999 Elsevier Science B.V. All rights reserved.