Enantiomeric separation of pirlindole by liquid chromatography using different types of chiral stationary phases

The enantioseparation of pirlindole by liquid chromatography (LC) was inves tigated using three different chiral stationary phases (CSPs) containing ei ther cellulose tris-(3,5-dimethylphenylcarbamate) (Chiralcel OD-R), ovomuco id (OVM) or beta-cyclodextrin (beta-CD). The effects of the: mobile phase p H on retention, enantioselectivity and resolution were studied. Methanol an d acetonitrile were tested, as organic modifiers while the influence of the addition to the mobile phase of sodium alkanesulfonates or sodium perchlor ate was also investigated. Sodium perchlorate was only used on the Chiralce l OD-R column while sodium alkanesulfonates were tested as mobile phase add itives on the three kinds of CSPs. The enantioseparation of pirlindole coul d be obtained on all CSPs tested, the best results with respect to chiral r esolution being achieved on the Chiralcel QD-R and the OVM columns. The use of sodium octanesulfonate (NaOS) was found to improve the enantioseparatio n of pirlindole on the OVM column while enantioselectivity was considerably enhanced by addition of sodium perchlorate on the Chiralcel OD-R column. ( C) 1997 Elsevier Science B.V. All rights reserved.