Gas chromatography mass spectrometric analysis of clenbuterol from urine

Clenbuterol which is mostly used as an anabolic agent. It is also used for treatment of asthma. Clenbuterol was analysed from urine by using gas chrom atography-mass spectrometry. GC;MS parameters were determined. Timolol was used as an internal standard. Extraction and derivatisation procedure of cl enbuterol from urine were developed. Clenbuterol was extracted by using die thylether/ter-butanol (4:1; v:v) and pH 12 K2CO3/KHCO (3:2; w:w) buffer. MS TFA/NH4I (1 ml/10 mg) mixture was used for derivatization of clenbuterol. S elected ions of clenbuteroll-bis-TMS were mit: 405, 337, 336, 335, 300, and 227. Extraction yield and minimum detection limit of clenbuterol from urin e were identified. Extraction yield was 94.30% and minimum detection was fo und 0.02 ng ml(-1) urine. It has been concluded that the GC-MS method is se nsitive, accurate, precise, and reproducible for analysing of clenbuterol f rom urine. (C) 1998 Elsevier Science B.V. All rights reserved.