COMPARATIVE IN-VITRO SENSITIVITY OF HUMAN CHOLANGIOCARCINOMA AND COLON ADENOCARCINOMA CELLS TO ANTICANCER AGENTS

We comparatively investigated the sensitivity, of a human cholangiocar cinoma cell line (SG231) and an adenocarcinoma cell line (WiDr) to mit oxantrone (MX) taxol (TX), mitomycin C (MMC), doxorubicin (DX), cispla tin (CDDP) and 5-fluorouracil (5FU) by the sulforhodamine B assay. The lower susceptibility of SG231 to 5FU, to CDDP, to DX and to MMC than WiDr was observed whereas the sensitivity of the two cell lines to MX and TX was similar. We also investigated the ability of a chemical mod ulator, lonidamine (LND), and hyperthermia to enhance the cytotoxic ac tivity of the different drugs in the SG231 cell line. No potentiation of MX or CDDP activity was observed after a 2 hours treatment in hyper thermic conditions (42 degrees C). Conversely, a slight potentiation o f a 2 hours pretreatment with MX and DX was obtained by a 24 hours pos t treatment with LND.