CHOLESTEROL BASED ANTINEOPLASTIC STRATEGIES

Manipulation of cholesterol metabolism open several possibilities of i nterfering with the growth of malignant cells. Deprivation of choleste rol decreases the velocity of growth and alters the composition of the cell membrane. The high requirement for LDL of malignant cells can be utilized for drug targeting Proliferation assays were performed with neuroblastoma cells and cell lines of acute myeloid leukemia deprived of cholesterol by inhibition of HMG-CoA-reductase or culture in LDL-de ficient medium. The cholesterol content of the cell membrane when I ed uced to 50 % had no effect on the toxicity of LAK-cells but the toxici ty of the fluorescent dye merocyanine MC 540 was enhanced two-fold. LD L-mediated drug targeting to AML cells was performed with oxidized LDL and showed toxic reactions. These results proved that cholesterol dep rivation could be used to support some therapeutic approaches.