Crystallization and preliminary X-ray diffraction analysis of group 5 miteallergen from Dermatophagoides pteronyssinus

Citation
Sh. Liaw et al., Crystallization and preliminary X-ray diffraction analysis of group 5 miteallergen from Dermatophagoides pteronyssinus, J STRUCT B, 123(3), 1998, pp. 265-268
Citations number
7
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF STRUCTURAL BIOLOGY
ISSN journal
10478477 → ACNP
Volume
123
Issue
3
Year of publication
1998
Pages
265 - 268
Database
ISI
SICI code
1047-8477(199811)123:3<265:CAPXDA>2.0.ZU;2-W
Abstract
Crystals of the 14-kDa group 5 allergen from Dermatophagoides pteronyssinus (Der p 5) have been obtained at low pH and diffract to 3-Angstrom resoluti on using a conventional x-ray source. The crystals belong to tetragonal spa ce group P4(1)2(1)2 or P4(3)2(1)2, with unit cell parameters a = b = 114 An gstrom and c = 234 Angstrom. A self-rotation search revealed a 432 point sy mmetry and thus suggested 96 molecules in one unit cell, hence 12 monomers in each asymmetric unit. (C) 1998 Academic Press.