Pg. Stevenson et Pc. Doherty, Non-antigen-specific B-cell activation following murine gammaherpesvirus infection is CD4 independent in vitro but CD4 dependent in vivo, J VIROLOGY, 73(2), 1999, pp. 1075-1079
The murine gammaherpesvirus MHV-68 multiplies in the respiratory epithelium
after intranasal inoculation, then spreads to infect B cells in lymphoid g
erminal centers, Exposing B cells to MHV-68 in vitro caused an increase in
cell size, up-regulation of the CD69 activation marker, and immunoglobulin
M (IgM) production. The infectious process in vivo was also associated with
increased CD69 expression on B cells in the draining lymph nodes and splee
n, together with a rise in total serum Ig. However, whereas the in vitro ef
fect on B cells was entirely T-cell independent, evidence of in vivo B-cell
activation was minimal in CD4(+) T-cell-deficient (I-A(b-/-)) or CD4(+) T-
cell-depleted mice. Furthermore, the Ig present at high levels in serum was
predominantly of the Ige class. Surprisingly, the titer of influenza virus
-specific serum IgG in previously immunized mice fell following MHV-68 infe
ction, suggesting that there was relatively little activation of memory B c
ells. Thus, CD4(+) T cells seemed both to amplify a direct viral activation
of B cells in lymphoid tissue and to promote new Ig class switching despit
e a lack of obvious cognate antigen.