F. Stevens et al., Computer modeling of atomic force microscopy force measurements: Comparisons of Poisson, histogram, and continuum methods, LANGMUIR, 15(1), 1999, pp. 207-213
A computer model of the AFM pull-off process was used to test the Poisson m
ethod for calculating bond forces and to compare the Poisson and histogram
methods of finding single-molecule bond-rupture forces. The Poisson method
appeared to work well, producing accurate individual bond-rupture forces ev
en in the presence of moderate to large amounts of different types of varia
tions or noise, while the histogram method only produced good results at lo
w noise levels, even with large sample sizes. Three cases were found where
use of the Poisson model could lead to inaccurate measurement of individual
bond forces, the most important being related to the bond-formation probab
ility, which must be low (ideally <5%) for accurate results. The Johnson-Ke
ndall-Roberts (JKR) continuum method was also examined. Poisson and JKR met
hods are not expected to produce similar results, but are complementary, an
d if used together may produce accurate measurements of both individual bon
d-rupture forces and bond-formation probabilities, even if neither method a
lone is successful.