Layer-by-layer construction of multilayer thin films composed of avidin and biotin-labeled poly(amine)s

Multilayer thin films composed of a positively charged protein and cationic polymer layers can be prepared on a solid surface against their electrosta tic force of repulsion, by depositing avidin and biotin-labeled polymers [p oly(ethyleneimine) (PEI), poly(allylamine) (PAA), or poly(amidoamine) (PAMA M) dendrimer] alternately and repeatedly. In contrast, the thin film does n ot form when unmodified polymers are used, confirming that the strong bindi ng between avidin and biotin is responsible for the formation of the multil ayer films. The loading of avidin in each layer of the multilayer films dep ends significantly on the molecular geometry of the polymers. The avidin/PA MAM dendrimer films are composed of a monomolecular layer of avidin whereas the avidin multilayers are formed in each layer of the PAA- and PEI-based thin films. The results are rationalized by the different molecular structu res of the polymers; PAMAM dendrimer assumes a globular shape, as compared with the linear and highly branched polymer chains of PAA and PEI, respecti vely.