Involvement of phospholipids in apolipoprotein B modification during low density lipoprotein oxidation

An increased amount of phospholipids remained attached on delipidated apoli poprotein B originated from oxidized low density lipoprotein (LDL). P-31 nu clear magnetic resonance analysis of such apolipoprotein showed an organic phosphorus peak at -0.55 ppm, which suggests the formation of adducts (most probably Schiff bases) of oxidized phospholipids with apolipoprotein B. Th e above reaction occurs in parallel with the hydrolysis of oxidized phospho lipids, catalyzed by the LDL-attached platelet-activating factor acetylhydr olase, and may contribute to the proatherogenic effect of oxidatively modif ied low density lipoprotein.