Evaluation of an enzyme-linked immunosorbent assay based on binding inhibition for type-specific quantification of poliovirus neutralization-relevantantibodies

To detect neutralization-relevant antibodies against 3 types of poliovirus (PV) without using tissue cultures and live viruses, an enzyme-linked immun osorbent assay (ELISA) based on monoclonal antibody-binding inhibition was evaluated using sera from 80 vaccinated Japanese children and 60 Pakistani poliomyelitis patients, Compared with the neutralization test, the sensitiv ity of the inhibition ELISA was 100% (111/111) for detection of anti-PV1 an tibody, 98.3% (118/120) for anti-PV2, and 96.5% (82/85) for anti-PV3, and t he specificity was 93.1% (27/29), 100% (20/20), and 92.7% (51/55), respecti vely. Thus, the inhibition ELISA showed excellent potential as a seroepidem iologic tool in both vaccinated and naturally-infected populations.