Focal cerebral ischemia in the mouse: description of a model and effects of permanent and temporary occlusion

Our study is to demonstrate the advantages and disadvantages of middle cere bral artery occlusion (MCAO) model in the mouse. CD-1 mice had permanent MC AO for 24 h, or temporary occlusion for either 1 h followed by 23 h of repe rfusion or 2 h of occlusion with 22 h of reperfusion. The infarct volume an d blood-brain barrier disruption were smaller in the 1-h/23-h temporary occ lusion than in either the 24-h permanent occlusion group or the 2-h/22-h te mporary occlusion group (p < 0.05). Our study demonstrates that blood flow, infarct volume, and Mood-brain barrier disruption remain important markers of focal cerebral ischemia. (C) 1999 Elsevier Science B.V. All rights rese rved.