An improved cocaine hydrolase: The A328Y mutant of human butyrylcholinesterase is 4-fold more efficient

Butyrylcholinesterase (BChE) has a major role in cocaine detoxication. The rate at which human BChE hydrolyzes cocaine is slow, with a k(cat) of 3.9 m in(-1) and K-m of 14 mu M. Our goal was to improve cocaine hydrolase activi ty by mutating residues near the active site. The mutant A328Y had a k(cat) of 10.2 min(-1) and K-m of 9 mu M for a 4-fold improvement in catalytic ef ficiency (k(cat)/K-m). Since benzoylcholine (k(cat) 15,000 min(-1)) and coc aine form the same acyl-enzyme intermediate but are hydrolyzed at 4000-fold different rates, it was concluded that a step leading to formation of the acyl-enzyme intermediate was rate-limiting. BChE purified from plasma of ca t, horse, and chicken was tested for cocaine hydrolase activity. Compared w ith human BChE, horse BChE had a 2-fold higher k(cat) but a lower binding a ffinity, cat BChE was similar to human, and chicken BChE had only 10% of th e catalytic efficiency. Naturally occurring genetic variants of human BChE were tested for cocaine hydrolase activity. The J and K variants (E497V and A539T) had k(cat) and K-m values similar to wild-type, but because these v ariants are reduced to 66 and 33% of normal levels in human blood, respecti vely, people with these variants may be at risk for cocaine toxicity. The a typical variant (D70G) had a 10-fold lower binding affinity for cocaine, su ggesting that persons with the atypical variant of BChE may experience seve re or fatal cocaine intoxication when administered a dose of cocaine that i s not harmful to others.