Expression profiling using cDNA microarrays

cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3 ' expressed sequence tags (ESTs), are arrayed onto glass slides (or membran es) and probed with fluorescent- or radioactively-labelled cDNAs. Here,we r eview technical aspects of cDNA microarrays, including the general principl es, fabrication of the arrays, target labelling, image analysis and data ex traction, management and mining.