Making and reading microarrays

There are a variety of options for making microarrays and obtaining microar ray data. Here, we describe the building and the use of two microarray faci lities in academic settings. In addition to specifying technical detail, we comment on the advantages and disadvantages of components and approaches, and provide a protocol for hybridization. The fact that we are now making a nd using microarrays to answer biological questions demonstrates that the t echnology can be implemented in university environment.