Substance P receptor (neurokinin-1)-expressing neurons in lamina I of the spinal cord encode for the intensity of noxious stimulation: A c-Fos study in rat

The substance P receptor neurokinin-l is expressed by a subset of neurons i n the rat spinal cord. We have combined immunostaining for Fos, a marker of noxious peripheral stimulation, and neurokinin-l to examine whether nocice ptive signals From particular peripheral tissues (skin, muscle or knee join t) or activity generated by nerve injury or formalin-induced inflammation a re preferentially modulated by substance P. Our results indicate that superficial and deep spinal neurokinin-1-positive neurons process nociceptive information in markedly different ways. In lam ina I, the number of double-labelled neurons was positively correlated with the intensity of the stimulus (defined by the total Fos count) and was not directly related to any particular peripheral target. However, in the deep er layers of the spinal cord (V-X), there was no such correlation, and stim ulation of joint nociceptors and formalin-induced inflammation produced the greatest proportion of Fos/neurokinin-1 co-localization, suggesting a part icular role for substance P in the mediation of joint pain and inflammatory hyperalgesia. Thus, lamina I neurokinin-l receptor-bearing neurons appear to be involved in intensity discriminative aspects of pain, whereas the deep neurokinin-l cells are involved in spatial localization or the detection of particular n ociceptive submodalities. (C) 1998 IBRO. Published by Elsevier Science Ltd.