Small polydisperse circular DNA (spcDNA) is a heterogeneous population of e
xtrachromosomal circular molecules present in a large variety of eukaryotic
cells. Elevated amounts of total spcDNA are related to endogenous and indu
ced genomic instability in rodent and human cells, We suggested spcDNA as a
novel marker for genomic instability, and speculated that spcDNA might ser
ve as a mutator, In this study, we examine the presence of telomeric sequen
ces on spcDNA, We report for the first time the appearance of telomeric rep
eats in spcDNA molecules (tel-spcDNA) in rodent and human cells. Restrictio
n enzyme analysis indicates that tel-spcDNA molecules harbor mostly, if not
exclusively, telomeric repeats, In rodent cells, tel-spcDNA levels are hig
her in transformed than in normal cells and are enhanced by treatment with
carcinogen, Tel-spcDNA is also detected in some human tumors and cell lines
, but not in others. We suggest, that its levels in human cells may be prim
arily related to the amount of the chromosomal telomeric sequences. Tel-spc
DNA may serve as a unique mutator, through specific mechanisms related to t
he telomeric repeats, which distinguish it from the total heterogeneous spc
DNA population. It may affect telomere dynamics and genomic instability by
clastogenic events, alterations of telomere size and sequestration of telom
eric proteins.