A rapid and efficient extraction method for onion DNA

Four DNA extraction protocols were investigated to identify a protocol that optimizes extraction of DNA from onion, Allium cepa, for amplification of DNA by polymerase chain reaction. The protocol selected was a simple proced ure which used fresh leaf tissue, resulted in high DNA yields and low DNA d egradation, low RNA contamination, was the least costly and required only 4 5 min. When RNAase T1 rather than RNAase A was used, more RNA was eliminate d.