Kanamycin is effective for selecting transformed peas

Authors
Grant, JE Cooper, PA Gilpin, BJ Hoglund, SJ Reader, JK Pither-Joyce, MD Timmerman-Vaughan, GM
Citation
Je. Grant et al., Kanamycin is effective for selecting transformed peas, PLANT SCI, 139(2), 1998, pp. 159-164
Citations number
13
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT SCIENCE
ISSN journal
01689452 → ACNP
Volume
139
Issue
2
Year of publication
1998
Pages
159 - 164
Database
ISI
SICI code
0168-9452(199812)139:2<159:KIEFST>2.0.ZU;2-W
Abstract
Immature cotyledons from six cultivars and breeding lines of Pisum sativum were cocultivated with Agrobacterium tumefaciens and selected on a medium c ontaining kanamycin. Transformed plants were recovered with a success rate of between 0.8 and 3.4% of explants treated. The plasmids used were based o n pGA633 and have a nos promoter driving an nptII gene. Progeny from the pr imary transformants showed stable inheritance of the nos-nptII gene. (C) 19 98 Elsevier Science Ireland Ltd. All rights reserved.