We have demonstrated a microfabricated single-molecule DNA sizing device. T
his device does not depend on mobility to measure molecule size, is 100 tim
es faster than pulsed-field gel electrophoresis, and has a resolution that
improves with increasing DNA length. It also requires a million times less
sample than pulsed-field gel electrophoresis and has comparable resolution
for large molecules. Here we describe the fabrication and use of the single
-molecule DNA sizing device for sizing and sorting DNA restriction digests
and ladders spanning 2-200 kbp.