A microfabricated device for sizing and sorting DNA molecules

Authors
Chou, HP Spence, C Scherer, A Quake, S
Citation
Hp. Chou et al., A microfabricated device for sizing and sorting DNA molecules, P NAS US, 96(1), 1999, pp. 11-13
Citations number
12
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
00278424 → ACNP
Volume
96
Issue
1
Year of publication
1999
Pages
11 - 13
Database
ISI
SICI code
0027-8424(19990105)96:1<11:AMDFSA>2.0.ZU;2-W
Abstract
We have demonstrated a microfabricated single-molecule DNA sizing device. T his device does not depend on mobility to measure molecule size, is 100 tim es faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single -molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.