Mf. Dreccer et al., SOURCE-SINK RATIO IN BARLEY (HORDEUM-VULGARE L) DURING GRAIN FILLING - EFFECTS ON SENESCENCE AND GRAIN PROTEIN-CONCENTRATION, Field crops research, 49(2-3), 1997, pp. 269-277
The impact of source-sink manipulations on plant senescence and grain
nitrogen (N) concentration was studied in barley plants (Hordeum vulga
re L.) by ear halving or partial defoliation after anthesis. Plants we
re sown in pots at two different dates (Experiment 1 and Experiment 2)
under ample N supply and were periodically detillered. In Experiment
1, plants were grown at similar temperature regime (15/9 degrees C) un
til 5 days after flowering, and then were subjected to either 15/9 deg
rees C or 22/16 degrees C during grain filling. The temperature treatm
ents were imposed to test if the effects of source-sink ratio on the c
haracteristics under study were independent of temperature during grai
n filling. The decrease in green leaf area and N concentration in stem
and leaves after flowering were insensitive to alterations in source-
sink ratio and similarly accelerated by temperature rise. The final we
ight of individual grains decreased only moderately with temperature r
ise and was not responsive to variation in the supply of assimilates.
Instead, reduction in ear size increased grain N concentration under a
ll environmental conditions, indicating that N accumulation in the gra
in. was source-limited in control plants. It appeared that the grain N
concentration necessary to achieve maximum grain weight was below tha
t which maximizes grain N accumulation. Grain N limitation by the sour
ce appeared after the initial phase of grain growth but was not eviden
t at other times during grain filling. The temporal changes in grain N
concentration in response to source-sink ratio during grain fill and
the potential significance of the results for modelling of grain N con
centration are discussed.