Effect of forage particle size and feeding frequency on fermentation patterns and sites and extent of digestion in dairy cows fed mixed diets

This trial aimed at studying the effects and possible interactions between forage particle size and feeding frequency on the site and extent of digest ion and ruminal fermentations throughout the day in dairy cows fed mixed di ets. Two forage particle sizes (chopped, C, or ground and pelleted, G, dehy drated alfalfa) and two feeding frequencies (two (2) or six (6) times daily ) were combined in a 2x2! arrangement. Four mid-lactation cows with cannula e in the rumen and proximal duodenum were used in a 4x4 Latin square design . They were fed at 95% of ad-libitum intake with three hours access to the manner after each meal distribution. On a dry-matter (DM) basis, the four d iets consisted of 40% dehydrated whole-crop maize, 4% long perennial ryegra ss hay, 25% dehydrated alfalfa and 31% concentrate (69% wheat). Dry-matter intake averaged 22.2 kg day(-1). No interaction between forage p article size and feeding frequency was found. Feeding G rather than C diets reduced chewing time (22.4 vs. 26.2 min kg(-1) DM), total and ruminal dige stibilities of organic matter (OM) and cell-wall constituents (p<0.10), and increased the non-ammonia nitrogen flow into the duodenum per unit of dige stible OM intake (p>0.05). Feeding G diets also reduced the molar proportio n of acetic acid and increased the molar proportions of propionic and valer ic acids. These changes in ruminal digestion were associated with a lower f ibrolytic activity and modifications in ruminal environment. The daily mean s of pH and ammonia concentration were decreased, while the range of pH dur ing the day and the time when pH and ammonia concentration were low (<6.0 a nd 2.9 mmol l(-1), respectively) were increased. At the same time, the prop ortion of fluid in the reticular contents and the fractional outflow rate o f liquids and small particles were decreased. Increasing feeding frequency did not affect carbohydrate digestion and mean ruminal parameters, although post-feeding variations of pH, osmolality and ammonia concentration were much less pronounced. It is concluded that reduced forage particle size can sharply affect the qu antity and the nature of digestive end-products by altering chemical and ph ysical conditions in the rumen. However, the magnitude of this effect is no t dependent on the amount of fermentable OM entering the rumen in one meal. (C) 1999 Elsevier Science B.V.