H. Inoue et al., GENOTOXIC EFFECT OF GRISEOFULVIN IN SOMATIC-CELLS OF DROSOPHILA-MELANOGASTER, Mutation research. Genetic toxicology testing, 343(4), 1995, pp. 229-234
Griseofulvin (GF), a carcinogenic spindle poison, was tested in two ty
pes of somatic-cell assays of Drosophila melanogaster, one of which de
tects the induction of DNA damage and the other mutation/mitotic recom
bination. In both assays, GF was fed to tester larvae and genetic endp
oints examined after emergence. In the wing spot test, trans-heterozyg
ous flies carrying mwh and flr(3) wing-hair mutations produced both si
gnificant and dose-dependent increases in the frequency of mwh single
spots over the control level but no increase of twin spots. In the DNA
repair test, double-mutant larvae carrying both mei-9(a) (excision re
pair-defective) and mei-41(D5) (postreplication repair-defective) muta
tions showed hypersensitivity to killing by GF compared with their DNA
repair-proficient counterparts, suggesting that GF caused potentially
lethal DNA damages which were efficiently repaired by the DNA repair-
proficient but not -defective larvae. These lines of evidence clearly
demonstrate that GF is genotoxic in somatic cells of Drosophila. It is
noted that (1) GF-fed larvae showed a developmental delay and (2) sur
viving adult flies had morphological abnormalities in their eyes and w
ings.