Kinetic model for the co-action of beta-amylase and debranching enzymes inthe production of maltose

The kinetics of the hydrolysis of starch with P-amylase and debranching enz ymes was studied. The hydrolysis of the alpha-1,6-glycoside bonds of the su bstrate by debranching enzymes does not create any new nonreducing ends, so debranching enzyme promotes the action of beta-amylase not by increasing t he concentration of the substrate of beta-amylase but by increasing the lin ear linkage portion of the substrate. The introduction of an effective chai n length function was used to formulate a kinetic model. (C) 1999 John Wile y & Sons, Inc.