Chronic inhibition of NOS does not prevent plasticity of rat somatosensory(S1) cortex following deafferentation

Nitric oxide (NO) has been proposed as an intercellular messenger mediating postsynaptic to presynaptic information transfer in the induction of long- term potentiation. A number of studies support the possible involvement of NO in synaptic plasticity. NO may have a role in synaptogenesis and synapti c plasticity in developing rat brain and may play a fundamental part in the process of regeneration, plasticity, and retargeting of axons following in jury. We examined the possible role of NO on plasticity in the rat first so matosensory cortex with [C-14]2-deoxyglucose (2-DG) autoradiography in rats treated daily with L-nitroarginine (L-NA) following neonatal unilateral vi brissae deafferentation. After 6 weeks of L-NA treatment, the local cerebra l glucose utilization (LCGU) and the spatial extent of the metabolic activa tion following stimulation of the spared whisker was measured. NOS catalyti c activity exhibited significant inhibition throughout the treatment period . Vibrissae deafferentation produced a small but not statistically signific ant increase of LCGU in the vibrissa activated C3 barrel, and L-NA treatmen t did not alter the activation of LCGU in the deafferented cortex following whisker stimulation. Additionally, L-NA treatment did not alter the area o f metabolic activation on either the non-deafferented side or the deafferen ted side. Deafferentation produced a 298% increase in the metabolic represe ntation of the spared C3 barrel following stimulation in the saline treated animals, a 257% increase in the chronically L-NA treated animals, and a 25 6% increase in the short-term treated animals, all with respect to the resp onse in the non-deafferented cortex. Metabolic plasticity in the barrel cor tex was not attenuated by L-NA treatment. These results show that nitric ox ide does not play a major role on developmental cortical plasticity induced by vibrissae deafferentation in the rat. (C) 1999 Elsevier Science B.V. Al l rights reserved.