Fabrication of amperometric biosensors by entrapment of enzymes in functionalized polypyrrole films

The concept and potentialities of an original electrochemical procedure of enzyme immobilization are described. This two-step method consists of the a dsorption of an aqueous amphiphilic pyrrole monomer-enzyme mixture on an el ectrode surface followed by the electropolymerization of the adsorbed monom ers. This procedure is compared with the conventional electrochemical metho ds of biosensor construction. Several bienzyme electrodes devoted to the im provement of the biosensor selectivity or to the amplification of the biose nsor sensitivity are described. In addition, examples of organic phase enzy me electrode and the electrical wiring of immobilized enzymes are presented .